von Wen Wang
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| [1.] Ww/Fragment 029 03 - Diskussion Zuletzt bearbeitet: 2014-10-30 08:32:57 Hindemith | Fragment, Gesichtet, Root et al 2006, SMWFragment, Schutzlevel sysop, Verschleierung, Ww |
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| Untersuchte Arbeit: Seite: 29, Zeilen: 3-11 |
Quelle: Root et al 2006 Seite(n): 716, Zeilen: figure 1 |
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Fig. 2. Map for the pLKO.1 lentiviral vector. It derives from pRRLSIN.cPPT.PGK/GFP/WRPE and contains elements for efficient viral packaging and shRNA expression. These include Ψ, the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/5’LTR is a hybrid of the Rous Sarcoma virus promoter and the HIV 5’LTR. SIN 3’LTR is the HIV 3’LTR with a self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter (hU6). The lentiviral vector also contains the mammalian selection marker puromycin resistance gene (PAC) under the control of the PGK promoter as a mammalian selection marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded phage F1 origin of replication, and AmpR is the ampicillin resistance gene. |
Figure 1 Vector map for the pLKO.1 lentiviral vector. The self-inactivating lentiviral vector backbone contains elements for efficient viral packaging and shRNA expression. These include Ψ, the lentiviral packaging site; RRE, the Rev-responsive element; cPPT. RSV/ 5′ LTR is a hybrid of the Rous Sarcoma virus promoter and the HIV 5′ LTR. SIN 3′ LTR is the HIV 3′ LTR with a self-inactivating U3 deletion. Expression of the shRNA is driven by the human U6 promoter (hU6). The lentiviral vector also contains the mammalian selection marker puromycin resistance gene (PAC) under the control of the PGK promoter as a mammalian selection marker. pUCori is the bacterial origin of replication of the plasmid, F1 ori is the single-stranded phage F1 origin of replication, and AmpR is the ampicillin resistance gene. |
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Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20141030083431