von Tanja Martens-Mantai
Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
| [1.] Tmm/Fragment 014 01 - Diskussion Zuletzt bearbeitet: 2014-04-27 00:35:23 Hindemith | El Harrak 2009, Fragment, Gesichtet, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Tmm |
|
|
|
| Untersuchte Arbeit: Seite: 14, Zeilen: 1-15 |
Quelle: El Harrak 2009 Seite(n): 12, Zeilen: 1ff |
|---|---|
| Material and methods
Adult rats (250–300 g) were decapitated under deep methohexital anaesthesia and the brains were rapidly removed to ice-cold (4 °C) artificial cerebrospinal fluid (ACSF). The cerebellum was removed and a cut was made to divide the two cerebral hemispheres. Combined amygdala-hippocampus–cortex slices containing the temporal cortex, the perirhinal cortex, the entorhinal cortex, the subiculum, the dentate gyrus, the hippocampus, as well as the amygdala (500 μm) were cut in a nearly horizontal plane. Up to two different slices from each side were collected in a preparation. Slices were stored at 28 °C in ACSF, which contained (in mm) NaCl, 124; KCl, 4; CaCl2, 1.0; NaH2PO4, 1.24; MgSO4, 1.3; NaHCO3, 26; glucose, 10 (pH 7.4), oxygenated with 95% O2 and 5% CO2 for > 1 h. After 30-min incubation, CaCl2 was elevated to 2.0 mmol/L. Slices were individually transferred to an interphase recording chamber, placed on a transparent membrane, illuminated from below and continuously perfused (1.5–2 mL/min) with carbogenated ACSF at 32 °C. A warmed, humified 95% O2 and 5% CO2 gas mixture was directed over the surface of the slices. |
Material and methods
Slice preparation Adult rats (250–300 g) were decapitated under deep methohexital anaesthesia and the brains were rapidly removed to ice-cold (4 °C) artificial cerebrospinal fluid (ACSF). The cerebellum was removed and a cut was made to divide the two cerebral hemispheres. Slices containing the temporal cortex, the perirhinal cortex, the entorhinal cortex, the subiculum, the dentate gyrus, the hippocampus, as well as the amygdala (500 μm) were cut in a nearly horizontal plane. Up to two different slices from each side were collected in a preparation. Slices were stored at 28 °C in ACSF, which contained (in mm) NaCl, 124; KCl, 4; CaCl2, 1.0; NaH2PO4, 1.24; MgSO4, 1.3; NaHCO3, 26; glucose, 10 (pH 7.4), oxygenated with 95% O2 and 5% CO2 for > 1 h. After 30-min incubation, CaCl2 was elevated to 2.0 mmol/L. Slices were individually transferred to an interphase recording chamber, placed on a transparent membrane, illuminated from below and continuously perfused (1.5–2 mL/min) with carbogenated ACSF at 32 °C. A warmed, humified 95% O2 and 5% CO2 gas mixture was directed over the surface of the slices. |
The source is not mentioned. |
|
| [2.] Tmm/Fragment 014 16 - Diskussion Zuletzt bearbeitet: 2014-04-27 01:38:52 Hindemith | Fragment, Gesichtet, Granz 2009, SMWFragment, Schutzlevel sysop, Tmm, Verschleierung |
|
|
|
| Untersuchte Arbeit: Seite: 14, Zeilen: 16-27 |
Quelle: Granz 2009 Seite(n): 16, Zeilen: 10-21 |
|---|---|
| Electrophysiological recordings
Extracellular field potentials were recorded with glass microelectrodes (150 mmol/l NaCl; 2–10 MΩ) connected to the amplifier by an Ag/AgCl–KCl bridge in the third layer of temporal neocortical tissues as well as in the entorhinal cortex and hippocampal CA1 and CA3 areas. Field potentials were traced by an ink-writer and recorded by a digital oscilloscope. Induction of neocortical SD SD was elicited by KCl microinjection. A glass electrode filled with 2 M KCl was fixed in a special holder connected with plastic tube to a pressure injector and the tip inserted into the sixth layer of the neocortical slices. A high-pressure pulse was applied to inject an amount of K+ in the tissue sufficient to induce cortical SD (tip diameter: 2 m; [sic] injection pressure 0.5–1.0 bar applied for 200-300 ms, two injections, 1–3 nl per pulse). |
1. Electrophysiological recordings
Extracellular field potentials were recorded with glass microelectrodes (150 mmol/l NaCl; 2–10 MΩ) connected to the amplifier by an Ag/AgCl–KCl bridge in the third and the fifth layers of neocortical tissues. Field potentials were traced by an ink-writer and recorded by a digital oscilloscope. 2. Induction of neocortical SD SD was elicited by KCl microinjection. A glass electrode filled with 2 M KCl was fixed in a special holder connected with plastic tube to a pressure injector and the tip inserted into the sixth layer of the neocortical slices. A high-pressure pulse was applied to inject an amount of K+ in the tissue sufficient to induce cortical SD (tip diameter: 2 m; [sic] injection pressure 0.5–1.0 bar applied for 200-300 ms, two injections, 1–3 nl per pulse). |
The source is not mentioned. |
|
Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20140427014130