von Dr. Tiziana Masullo
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| [1.] Tim/Fragment 023 03 - Diskussion Zuletzt bearbeitet: 2014-10-27 04:52:36 Hindemith | Fragment, Gesichtet, Isarankura 2009, SMWFragment, Schutzlevel sysop, Tim, Verschleierung |
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| Untersuchte Arbeit: Seite: 23, Zeilen: 3-5 |
Quelle: Isarankura 2009 Seite(n): 353, Zeilen: last lines |
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| Engineered E. coli co-expressing surface exposed zinc-binding motif and GFP has been applied for real-time monitoring of intracellular mobility of zinc ions (Isarankura-Na-Ayudhya et al., 2005).
Isarankura-Na-Ayudhya C., Suwanwong Y., Boonpangrak S., Kiatfuengfoo R., Prachayasittikul V., 2005. Co-expression of zinc binding motif and GFP as a cellular indicator of metal ions mobility. Int. J. Biol. Sci., 1: 146-151. |
Additionally, engineered E. coli co-expressing surface exposed zinc-binding motif and GFP has been applied for real-time monitoring of intracellular mobility of zinc ions [27].
27. Isarankura-Na-Ayudhya C, Suwanwong Y, Boonpangrak S et al (2005) Co-expression of zinc binding motif and GFP as a cellular indicator of metal ions mobility. Int J Biol Sci 1:146–151 |
The source given does not contain the copied text. |
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| [2.] Tim/Fragment 023 19 - Diskussion Zuletzt bearbeitet: 2014-10-27 04:13:04 Hindemith | BauernOpfer, Fragment, Fu 2008, Gesichtet, SMWFragment, Schutzlevel sysop, Tim |
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| Untersuchte Arbeit: Seite: 23, Zeilen: 19-26 |
Quelle: Fu 2008 Seite(n): abstract, p.6, Zeilen: p.6: last lines |
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| In the study of 2008, Fu et al. observed the proximity of single GFPs to metallic silver nanoparticles increases its fluorescence intensity approximately 6-fold and decreases the decay time. Single protein molecules on the silvered surfaces emitted 10-fold more photons as compared to glass prior to photobleaching. The photostability of single GFP has increased to some extent. Accordingly, we observed longer duration time and suppressed blinking. The detection of metal-surface enhanced fluorescence from GFP suggests the more extensive use of metallic nanostructures in imaging and single molecule detection. | In this report, we observed the proximity of single GFPs to metallic silver nanoparticles increases its fluorescence intensity approximately 6-fold and decreases the decay time. Single protein molecules on the silvered surfaces emitted 10-fold more photons as compared to glass prior to photobleaching. The photostability of single GFP has increased to some extent. Accordingly, we observed longer duration time and suppressed blinking.
The detection of metal-surface enhanced fluorescence from GFP suggests the more extensive use of metallic nanostructures in imaging and single molecule detection. |
The source is given, but it is not clear to the reader how much has been taken from it. In particular the two sentences starting with "Accordingly, we observed" cannot be attributed to the source by the reader who rather assumes that the author herself made observations that are consistent with the findings of the source. |
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Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20141027041636