Rlm/Fragment 054 11

Typus KomplettPlagiat

Bearbeiter Graf Isolan

Gesichtet

Untersuchte Arbeit: Seite: 54, Zeilen: 11-17

Quelle: Rizzolio 2010 Seite(n): 60, Zeilen: 12-18

Lentiviral production To generate knock down cells, lentiviral particles were produced as described (http://www.broadinstitute.org/genome_bio/trc/publicProtocols.html). Briefly, 1x106 293FT cells (Invitrogen Corp, Carlsbad, CA, USA) were transfected with 2.25 μg of PAX2 packaging plasmid, 0.75 μg of PMD2G envelop plasmid and 3μg of pLKO.1 hairpin vector utilizing [30 μl of Fugene HD (Roche Applied Science, Indianapolis, IN, USA) in 10 cm plate.]

Lentiviral production To generate knock down cells, lentiviral particles were produced as described (http://www.broadinstitute.org/genome_bio/trc/publicProtocols.html). Briefly, 1x106 293FT cells (Invitrogen Corp, Carlsbad, CA, USA) were transfected with 2.25 μg of PAX2 packaging plasmid, 0.75 μg of PMD2G envelop plasmid and 3μg of pLKO.1 hairpin vector utilizing 30 μl of Fugene HD (Roche Applied Science, Indianapolis, IN, USA) in 10 cm plate.

Anmerkungen Although identical, nothing has been marked as a citation. Continued on the next pages.

Sichter (Graf Isolan), SleepyHollow02