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| Untersuchte Arbeit: Seite: 35, Zeilen: 9-17 |
Quelle: Rizzolio 2010 Seite(n): 45, Zeilen: 7 ff. |
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| In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. It is fused to the N-terminus of a protein. Agarose beads can be coated with glutathione, and such glutathione-Agarose beads bind GST-proteins. These beads are then washed, to remove contaminating bacterial proteins. | In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. It is fused to the N-terminus of a protein. Agarose beads can be coated with glutathione, and such glutathione-Agarose beads bind GST-proteins. These beads are then washed, to remove contaminating bacterial proteins. |
Although identical, nothing has been marked as a citation. No source given. |
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