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Titel | Cortisol analysis laboratory |
Herausgeber | Department of Psychology, NUI Galway |
Ort | Galway |
Datum | 5. December 2006 |
Anmerkung | The date is inferred from the document properties of the linked word file. |
URL | http://www.nuigalway.ie/psychology/documents/Cortisol_analysis_laboratory.doc |
Webcite | http://www.webcitation.org/6NUGYbYPK |
Literaturverz. |
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Fußnoten | no |
Fragmente | 2 |
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[1.] Jm/Fragment 097 17 - Diskussion Zuletzt bearbeitet: 2014-01-17 20:40:08 Hindemith | Fragment, Gesichtet, Jm, NUI Galway 2006, SMWFragment, Schutzlevel sysop, Verschleierung |
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Untersuchte Arbeit: Seite: 97, Zeilen: 17-22 |
Quelle: NUI Galway 2006 Seite(n): 2, Zeilen: 2-7 |
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Salivette swabs are available with a citric acid infusion to stimulate saliva flow, but the citric acid is liable to affect pH levels of samples, and is thus to be avoided. Since the untreated pure cotton swabs, employed herein, supplied with standard Salivette tubes usually collect a sound sample of 1½ - 2 ml of saliva after between 60-80s of chewing, obtaining a sufficient sample is usually unproblematic (Kirschbaum & Hellhammer, 2000). | SalivetteTM swabs are available with a citric acid infusion to stimulate saliva flow, but the citric acid is liable to affect pH levels of samples, and is thus to be avoided. Since the untreated pure cotton swabs supplied with standard SalivetteTM tubes usually collect a sound sample of 1½ - 2 ml of saliva after between 60-80s of chewing, obtaining a sufficient sample is usually unproblematic. |
The source is not referenced. |
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[2.] Jm/Fragment 099 03 - Diskussion Zuletzt bearbeitet: 2014-02-18 20:49:30 Hindemith | Fragment, Gesichtet, Jm, NUI Galway 2006, SMWFragment, Schutzlevel sysop, Verschleierung |
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Untersuchte Arbeit: Seite: 99, Zeilen: 3-7, 9-11, 12-17, 20-21 |
Quelle: NUI Galway 2006 Seite(n): 1, Zeilen: 21-35, 38-40 |
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Unstimulated (i.e., presumably unaffected by stressors or other factors – ‘normal’/baseline level) cortisol follows a diurnal rhythm that is dictated by the sleep-wake cycle, rather than a light-dark cycle: there is a typical and consistent flood of cortisol in the body upon awakening, generally declining thereafter, and cortisol secretion follows a series of peaks and troughs throughout the day, [with a small peak associated with a lunch-time meal (Preussner et al., 1997, in Pollard & Ice, 2007; Kirschbaum & Hellhammer, 2000),] which tends to taper off to a more steady, less steep decline in the afternoon. It is for this reason that laboratory studies examining cortisol generally schedule post-noon testing sessions. [Therefore, all participants were scheduled to commence the session at 3pm in the current study (see Appendix 14).] Variations in this cycle are seen in clinical populations – for instance, a blunted response has been demonstrated in individuals with depression and those experiencing socioeconomic hardship, while extremely elevated, or again, blunted cortisol release can result from a variety of medical conditions. Cortisol secretion can be affected by everyday factors such as consumption of food or beverages prior to sampling, or disrupted sleep patterns. [As a result, stringent guidelines were set forth to control for these factors, and various control measures were distributed (see above) to screen for possible psychopathologies (see Appendices 10 and 11).]
Cortisol in saliva is a particularly robust biologically-active compound, and samples remain viable for several days at room temperature, as well as when frozen and refrozen. |
Unstimulated (i.e., presumably unaffected by stressors or other factors – ‘normal’/baseline level) cortisol follows a diurnal rhythm that is dictated by the sleep-wake cycle, rather than a light-dark cycle: there is a typical and consistent flood of cortisol in the body upon awakening, generally declining thereafter, and cortisol secretion follows a series of peaks and troughs throughout the day, which tend to taper off to a more steady, less steep decline in the afternoon. It is for this reason that laboratory studies examining cortisol usually schedule post-noon testing sessions. Variations in this cycle are seen in clinical populations – for instance, a blunted response has been demonstrated in individuals with depression and those experiencing socioeconomic hardship, while extremely elevated, or again, blunted cortisol release can result from a variety of medical conditions. Cortisol secretion can be affected by everyday factors such as consumption of food or beverages prior to sampling, or disrupted sleep patterns. [Clinically, hyper-secretion of cortisol can lead to the development of Cushing’s disease. while hypo-secretion can result in Addison’s disease.]
Cortisol in saliva is a particularly robust biologically-active compound, and samples remain viable for several days at room temperature, as well as when frozen and refrozen. |
No source is given. |
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