von Dr. Pawandeep Kaur
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| [1.] Pak/Fragment 033 01 - Diskussion Zuletzt bearbeitet: 2014-04-06 06:49:11 Hindemith | Ahmed 2007, Fragment, Gesichtet, Pak, SMWFragment, Schutzlevel sysop, Verschleierung |
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| Untersuchte Arbeit: Seite: 33, Zeilen: 1-3, 6-21 |
Quelle: Ahmed 2007 Seite(n): 33-34, Zeilen: 33: 1-2,14-18, 26-30 - 34: 1-8 |
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[NOD/SCID mice were bred from the breeding] pairs originally obtained from Taconic Bomholt, Denmark and maintained in the animal facility located at the GSF-Haematology, Munich. All animals were handled under sterile conditions and maintained under micro isolators. [xenografts [sic] into immunodeficient NOD-SCID mice are the gold standard readout for human long-term repopulating hematopoietic cells.110-112]
Sterile Syringes: BD Plastipak 1 ml syringe (BD Biosciences, Palo Alto, CA) for injection of cells in mice and Kendall Monoject 3 ml syringes (Tyco Healthcare, UK) for bone marrow flushing and plating of CFC. Sterile needles: 0.5 x 25 mm for intra venous injection of cells in mice and 0.55 x 25 mm (BD Microlance, Drogheda, Ireland) for bone marrow aspiration from living mice and flushing of bone marrow from extracted bones. 16 X 1.5 inch needles for dispensing and plating Methocult (CFC) media (Stem Cell Technologies, Vancouver, Canada). Ammonium Chloride solution: For erythrocyte lysis 0.8% NH4Cl with 0.1 mM EDTA (Stem Cell Technologies, Vancouver, Canada). Heparinized capillaries: (Microvette CB 300) plastic capillaries for collection of blood, containing 15 units (I.E) Lithium heparin per ml of blood (Sarstedt, Numbrecht, Germany).
• E. coli DH5á [sic] |
3.1.5 Bacterial strain
• E. coli DH5α [...] NOD/SCID mice were bred from the breeding pairs originally obtained from Taconic Europe and maintained in the animal facility located at the GSF-Haematology, Munich. All animals were handled under sterile conditions and maintained under micro isolators. 3.1.7 Mice related reagents and equipment: [...] Sterile Syringes: BD Plastipak 1 ml syringe (BD Biosciences, Palo Alto, CA) for injection of cells in mice and Kendall Monoject 3 ml syringes (Tyco Healthcare, UK) for bone marrow flushing and plating of CFC. Sterile needles: 0.5 x 25 mm for intra venous injection of cells in mice and 0.55 x 25 mm (BD Microlance, Drogheda, Ireland) for bone marrow aspiration from living [page 34] mice and flushing of bone marrow from extracted bones. 16 X 1.5 inch needles for dispensing and plating Methocult (CFC) media (Stem Cell Technologies, Vancouver, Canada) Ammonium Chloride solution: For erythrocyte lysis 0.8% NH4Cl with 0.1 mM EDTA (Stem Cell Technologies, Vancouver, Canada) Heparinized capillaries: (Microvette CB 300) plastic capillaries for collection of blood, containing 15 units (I.E) Lithium heparin per ml of blood (Sarstedt, Numbrecht, Germany). |
The source is not given. |
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Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20140406070617