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| [1.] Iam/Fragment 025 01 - Diskussion Zuletzt bearbeitet: 2014-03-12 20:16:29 Graf Isolan | Fragment, Gesichtet, Iam, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Sheikh 2006 |
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| Untersuchte Arbeit: Seite: 25, Zeilen: 1ff (complete) |
Quelle: Sheikh 2006 Seite(n): 25, Zeilen: 3-14 |
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| 3.1.2 Isolation of rat hepatocytes and irradiation
Hepatocytes were isolated from male Wistar rats by circulating perfusion with collagenase essentially as described previously (Seglen 1972). 3.1.2.a. Liver perfusion After laparotomy, the vena portae was canulated, vena cava inferior was ligated above the diaphragm to prevent flow of the perfusion media into a whole body circulation. Finally, the vena cava inferior was cut beneath the liver and canulated. The liver was perfused in non-recirculative mode through the portal vein with 150-200 ml CO2-enriched preperfusion medium at a flow rate of 30 ml/min until the liver was free from blood. To break down components of extracellular matrix, the liver was perfused in recirculative mode with collagenase perfusion medium until it started to feel soft (about 7-11 min). Seglen PO (1972) Preparation of rat liver cells. I. Effect of Ca 2+ on enzymatic dispersion of isolated, perfused liver. Exp Cell Res 74:450-454 |
3.1.1 Isolation of rat hepatocytes
Hepatocytes were isolated from male Wistar rats by circulating perfusion with collagenase essentially as described previously (Seglen, 1973; Katz et al., 1979). 3.1.1.I Liver perfusion After laparotomy, the vena portae was canulated, vena cava inferior was ligated above the diaphragm to prevent flow of the perfusion media into a whole body circulation. Finally, the vena cava inferior was cut beneath the liver and canulated. The liver was perfused in non-recirculative mode through the portal vein with 150-200 ml CO2-enriched preperfusion medium at a flow rate of 30 ml/min until the liver was free from blood. To break down components of extracellular matrix, the liver was perfused in recirculative mode with collagenase perfusion medium until it started to feel soft (about 7-11 min). |
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Letzte Bearbeitung dieser Seite: durch Benutzer:Graf Isolan, Zeitstempel: 20140312202446