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| Untersuchte Arbeit: Seite: 55, Zeilen: 3-13 |
Quelle: Boini 2006 Seite(n): 34, Zeilen: 4ff |
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| Mice deficient in SGK1 (sgk1–/–) were generated and bred as previously described (Huang et al., (2004) J Am Soc Nephol; Wulff et al., (2002) J Clin Invest; Huang et al., (2004) J Am Soc Nephol). In brief, a conditional targeting vector was generated from a 7-kb fragment encompassing the entire transcribed region on 12 exons. The neomycin resistance cassette was flanked by two loxP sites and inserted into intron 11. Exons 4–11, which code for the sgk1 kinase domain, were “floxed” by inserting a third loxP site into intron 3. A clone with a recombination between the first and third loxP site (type I recombination) was injected into C57BL/6 blastocytes. Male chimeras were bred to C57BL/6 and 129/SvJ females. Heterozygous SGK1-deficient mice were backcrossed to 129/SvJ wild-type mice (Charles River, Sulzfeld, Germany) for ten generations and then intercrossed to generate homozygous SGK1 knockout mice (sgk1−/−) and their wild type littermates (sgk1+/+). | Mice deficient in SGK1 (sgk1-/-) were generated and bred as previously described (Wulff et al., 2002; Huang et al., 2004). In brief, a conditional targeting vector was generated from a 7-kb fragment encompassing the entire transcribed region on 12 exons. The neomycin resistance cassette was flanked by two loxP sites and inserted into intron 11. Exons 4–11, which code for the sgk1 kinase domain, were “floxed” by inserting a third loxP site into intron 3. A clone with a recombination between the first and third loxP site (type I recombination) was injected into C57BL/6 blastocytes. Male chimeras were bred to C57BL/6 and 129/SvJ females. Heterozygous SGK1-deficient mice were backcrossed to 129/SvJ wild-type mice (Charles River, Sulzfeld, Germany) for ten generations and then intercrossed to generate homozygous SGK1 knockout mice (sgk1-/-) and their wild type littermates (sgk1+/+). |
The source is not mentioned. It is not surprising that the two dissertations have used mice that have been treated identically, and it is efficient to describe this with the same words, but this should have been made transparent. |
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