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| Untersuchte Arbeit: Seite: 114, Zeilen: 3-13 |
Quelle: Opitz et al 2004 Seite(n): 719, 722, Zeilen: 719: right col. 14-22; 722: right col. 19ff |
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| The binding of DNA onto patterned aminoterpolymer film and these investigation allowed us to answer the question of whether deprotected amino group are exposed to the surface upon UV-irradiation to obtain a strong binding with DNA. This highly site-specific binding of DNA to the patterned aminoterpolymer layer showed in general that patterned aminofilm are well suited for a site-specific immobilization of negatively changes biomolecules. In this approach the patterning shown not only changes in chemistry but also with regard to changes in surface charge. The surface charge of deprotected aminofilms areas can be tuned to obtain a highly selective, end-specific DNA binding. The obtained binding forces are large enough to allow single molecule manipulation of DNA which is one important prerequisite for biological or bio-physical studies at single molecule level. | [page 719]
These investigations allow us to answer the question of whether deprotected amino groups are exposed to the surface upon UV irradiation with a sufficient high density to obtain a strong electrostatic binding of charged nanoparticles. Within this approach the patterning will be characterized not only regarding changes in chemistry, which could preferentially or even solely happen ‘inside’ the — compared to SAMs — relatively thick ATP films, but also with regard to changes in surface charge. [page 722] 4. Conclusions [...] A highly site-specific binding of DNA to the patterned aminoterpolymer layer was observed, which shows — in general — that patterned ATP films are well suited for a site-specific immobilization of negatively charged biomolecules. The surface charge of the deprotected ATP-film areas can be tuned to obtain a highly selective, end-specific DNA binding. The obtained binding forces are large enough to allow single-molecule manipulation of DNA which is one important prerequisite for biological or bio-physical studies at single-molecule level. |
From the Result and Discussion-part of Bsi's thesis. First slightly adapted, later nearly identical. No source given. Nothing has been marked as a citation. Passage stems from a paper originated by the research group of which Bsi was a member. Bsi is not named as a co-author or as a participator in the research being described in the paper. |
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