|
|
|
| Untersuchte Arbeit: Seite: 42, Zeilen: 2-8 |
Quelle: Wikipedia Peptide synthesis 2007 Seite(n): 1 (internet resource), Zeilen: - |
|---|---|
| In order to remove BOC from a growing peptide chain acidic conditions (usually TFA) are necessary. Removal of side-chain protecting groups and the peptide from the resin at the end of the synthesis is achieved by incubating in hydrofluoric acid. For this reason BOC chemistry is generally disfavoured. However, when synthesizing nonnatural peptide analogy which are base-sensitive (such as depsi-peptides), t-BOC is necessary. | Boc SPPS
When R. B. Merrifield invented SPPS in 1963, it was according to the tBoc method. t-Boc (or Boc) stands for (tert)-(B)utyl (o)xy (c)arbonyl. To remove Boc from a growing peptide chain, acidic conditions are used (usually neat TFA). Removal of side-chain protecting groups and the peptide from the resin at the end of the synthesis is achieved by incubating in hydrofluoric acid (which can be dangerous); for this reason Boc chemistry is generally disfavored. However for complex syntheses Boc is favourable. When synthesizing nonnatural peptide analogs which are base-sensitive (such as depsipeptides), Boc is necessary. |
Nothing has been marked as a citation. |
|